standard substance p release curve Search Results


substance p  (Revvity)
91
Revvity substance p
Substance P, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard substance p release curve  (Tocris)
95
Tocris standard substance p release curve
Standard Substance P Release Curve, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic substance p standard  (Millipore)
90
Millipore synthetic substance p standard
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Synthetic Substance P Standard, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substance p  (Bachem)
90
Bachem substance p
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Substance P, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flagtm peptide  (Kodak)
90
Kodak flagtm peptide
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Flagtm Peptide, supplied by Kodak, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sar 9 ,met(o 2 ) 11 )-substance p  (Bachem)
90
Bachem sar 9 ,met(o 2 ) 11 )-substance p
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Sar 9 ,Met(O 2 ) 11 ) Substance P, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag peptide  (Kodak)
90
Kodak flag peptide
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Flag Peptide, supplied by Kodak, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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urotensin ii  (Bachem)
90
Bachem urotensin ii
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Urotensin Ii, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substance p  (AnaSpec)
90
AnaSpec substance p
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Substance P, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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senktide  (Bachem)
90
Bachem senktide
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Senktide, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substance p, rat monoclonal igg2a antibody  (Millipore)
90
Millipore substance p, rat monoclonal igg2a antibody
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Substance P, Rat Monoclonal Igg2a Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substance p  (Leeman Labs)
90
Leeman Labs substance p
<t>Substance</t> <t>P</t> release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.
Substance P, supplied by Leeman Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Substance P release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.

Journal:

Article Title: Sufentanil, Morphine, Met-enkephalin, and ?-Agonist (U-50,488H) Inhibit Substance P Release from Primary Sensory-Neurons: A Model for Presynaptic Spinal Opioid Actions

doi:

Figure Lengend Snippet: Substance P release during three phases of electrical field stimulation: Diagrams showing the result of a typical experiment. Nine cultures from a single plating were divided into three groups of three as shown in table 1. Each culture was stimulated for 3 min at 1 Hz. Baseline (shaded bars) and evoked (blanked bars) levels of substance P immunoreactivity were determined by direct RIA of the release buffer for three repeated phases of stimulation. S1, 2, and 3 stand for the amount of substance P released in Phases 1, 2, and 3, respectively. The upper diagram shows results for the external control group. In the absence of any drug, the amount of evoked substance P release was not significantly different for the three repeated phases. The middle diagram shows the result of the agonist-treated (sufentanil 0.5 µm) group. The presence of sufentanil during Phase 2 inhibited the evoked substance P release by 54% (P < 0.05) as compared with Phase 1 and 3 of the same group. The lower diagram shows the result of the agonist/antagonist-treated (sufentanil 0.5 µm and naloxone 25 µm) group. Naloxone completely blocked the inhibitory effect of sufentanil. Error bars indicate the mean ± SD (n = 3) for released substance P as determined for three cultures of a single group.

Article Snippet: Standard curves were generated by assaying serial dilutions of a synthetic substance P standard (Sigma) that was diluted in the same release buffer as was used for the release experiment.

Techniques: